Core Technology Disclosure

Introduction: Originating from Life, Optimizing Life – The Wisdom of Biomimetic Regenerative Medicine

The Science Behind Our Revolutionary Regenerative Material

Biomimetics, the practice of imitating principles from biological systems to construct technical systems, holds an unparalleled core position and vast potential in regenerative medicine. Our design philosophy for biomimetic regenerative materials is to "learn from nature and optimize nature." By accurately simulating and optimizing the structural and functional characteristics of the human body's own extracellular matrix (ECM), we aim to create a microenvironment that actively guides, efficiently promotes, and safely enables tissue regeneration, thereby achieving "true" tissue repair and functional reconstruction.

Material Composition & Core Engine: The Extraordinary Characteristics of Soluble Collagen

This biomimetic regenerative material takes high-purity "soluble collagen" as its absolute core component. Collagen is the main structural protein in animal connective tissue, accounting for 25%-30% of the total protein in mammals. It is predominantly found in human structural tissues:

• Tendons and ligaments (approx. 70-85% collagen)
• Cornea (containing over 90% collagen)
• Blood vessels (Mainly Type I and III collagen, 50-70%)
• Gums (50-65%)
• Visceral liver matrix (15-20%)
• Kidney matrix (approx. 10-15%)
• Gastrointestinal tract (approx. 25-30%)
• Fascia (60-80%)
• Uterus (40-50%)
• Tracheal and bronchial wall (30-40%)
• Nerve sheath (20-25%)
• Heart valve (50-60%)
• Gallbladder wall (35-40%)
• Breast tissue (20-30%)

This collagen biomimetic material possesses excellent mechanical strength, low antigenicity, and high affinity, enabling it to simulate  tissues and effectively repair or regenerate these tissues. As a biomedical material, it has many inherent advantages:

• Excellent Biocompatibility: Natural affinity with human tissues, minimal immune rejection or adverse inflammatory reactions.
• Controllable Biodegradability: Enzymatically decomposed and gradually absorbed; degradation products (e.g., amino acids) are non-toxic and reusable by the body. Degradation rate is tunable via cross-linking to match different tissue regeneration cycles.
• Extremely Low Immunogenicity: Minimized in highly purified and specifically treated collagen, especially soluble collagen, reducing post-implantation risks.
• Superior Cell Adhesion & Proliferation Support: Contains RGD and other cell recognition sequences, promoting cell adhesion, migration, proliferation, and differentiation, providing an ideal "soil" for tissue regeneration.

We specifically choose collagen in its "soluble" form, which brings additional unique advantages:

• Ease of Purification & Processing: Soluble collagen allows for easier removal of impurities and potential immunogenic substances, yielding high-purity raw materials. Its solution state facilitates processing into complex 3D scaffolds with specific microstructures and macro forms using advanced technologies like freeze-drying, electrospinning, and 3D printing.
• Formation of Complex 3D Network Structures: Under certain conditions, soluble collagen can self-assemble to form a fiber network structure similar to natural ECM, providing three-dimensional growth space for cells.
• Facilitates Loading of Bioactive Molecules: Convenient for incorporating growth factors, drugs, or other bioactive molecules during material preparation for controlled release, further optimizing the regenerative microenvironment.
• Enhanced Material-Host Tissue Integration: Soluble collagen interacts more readily with the host tissue's ECM, promoting seamless integration of new tissues with surrounding healthy tissues.

Quality & Safety: We strictly control the source of collagen (e.g., medical-grade animal tissue or genetic engineering recombinant expression) and adopt advanced purification technology and virus inactivation treatments to ensure high material safety and the stability and reliability of biological properties.

Exquisite Biomimetic Design: Triple Simulation and Transcendence of Composition, Structure, and Function

The biomimetic design of this material is reflected at multiple levels—microstructure, macromorphology, and biological function—aiming to provide a "home-like" regenerative environment for cells.

Component Biomimetics:

• Tendon Example: Natural tendons are primarily Type I collagen (80% dry weight), forming tight fiber bundles for tensile strength. Minor amounts of Type III, V collagen, elastin (2%), proteoglycans, and fibronectin contribute to structure, flexibility, and repair. Our artificial tendons, mainly using soluble Type I collagen, highly mimic this composition.
• Compositional Matching: At interfaces like tendon-muscle or tendon-bone junctions, we adjust collagen concentration (gradient), cross-linking degree, and compound with other biocompatible materials to match the target natural tissue.

Structural Biomimetics:

• Microstructure: Load-bearing tissues (fascia, tendons, ligaments, muscles) often consist of parallel fiber bundles. Our collagen threads, made from soluble collagen, are composed of many tiny collagen fibers, providing great mechanical strength. Weaving these threads into bundles creates structures very similar to tendon/ligament muscle tissue. Precise control of preparation processes (e.g., freeze-drying rate/temperature, electrospinning voltage/distance) constructs 3D structures (tens to hundreds of microns) simulating natural ECM topology. This facilitates uniform cell infiltration, nutrient/oxygen transport, and waste removal. Fiber diameter, orientation (e.g., for tendon/nerve regeneration), and surface morphology are carefully designed to guide cell behavior.
• Macrostructure & Mechanical Matching: Materials are prepared in various macroforms (membrane, tubular, sponge, fiber bundle) according to repair needs. Crucially, we match mechanical properties (tensile strength, elastic modulus, toughness, compressive strength) with the target natural tissue by regulating collagen concentration, cross-linking, and compounding with biocompatibility enhancers. For instance, tendon regeneration materials require high tensile strength and appropriate elasticity, while nerve regeneration conduits need flexibility and anti-collapse ability. This mechanical matching provides physical support and promotes cell differentiation and tissue maturation via mechanotransduction.

Functional Biomimetics:

• Mechanical Strength Simulation: The implant material itself possesses high strength, fulfilling the load-bearing function of tissues like fascia, tendons, and ligaments.
• Biosignal Simulation: Collagen and its degradation peptides provide biochemical signals (e.g., RGD sequences) similar to natural ECM, directly regulating cell adhesion, proliferation, migration, and differentiation. We can further simulate complex signal environments by loading specific growth factors (e.g., VEGF for vascularization, NGF for nerve regeneration, TGF-β for cartilage formation).
• Dynamic Microenvironment Construction: The material's biodegradation rate is designed to coordinate with new tissue formation. Initially, it provides stable structural support and growth-promoting signals; as new tissue matures, the material degrades synchronously, making space and eventually being fully absorbed, avoiding long-term complications of permanent implants. This "degradation and regeneration" dynamic is key to ideal tissue repair.

Figure 1 

Incomparable Advantages: Why Our Technology is Subversive

Compared to existing treatments, our soluble collagen-based biomimetic regeneration materials exhibit remarkable, multifaceted advantages:

• Extreme Biocompatibility & Safety: Extensive animal experimental data prove no cytotoxicity, no systemic toxic reactions, and extremely low immunogenicity post-implantation.
• Strong Regeneration Promotion & Tissue Guidance Ability: Effectively recruits host stem/progenitor cells to the defect site, provides an ideal microenvironment for their proliferation, differentiation, and ECM synthesis, promotes rapid neovascularization and nerve re-innervation, and guides orderly tissue regeneration.
• Customizable Physicochemical Characteristics: Precise regulation of degradation rate, mechanical properties (strength, elasticity, toughness), pore structure, and macromorphology by adjusting collagen concentration, cross-linking methods, composite components, and molding processes to suit various soft tissue defects.
• Excellent Tissue Integration & Functional Reconstruction: Regenerated tissue achieves seamless integration with host healthy tissue, gradually restoring the original fine structure and physiological function, rather than simple scar filling.
• Good Nutrient Penetration & Metabolite Exchange: Carefully designed porous structure ensures cells receive sufficient nutrient supply and oxygen, and can efficiently discharge metabolic waste, ensuring cell survival and tissue growth.

Advanced Manufacturing Technology

We employ a series of innovative or optimized integrated advanced manufacturing processes: highly selective soluble collagen extraction and purification, precisely controlled cross-linking technology (to regulate degradation rate and mechanical properties), specific 3D structure molding processes (e.g., programmatic freeze-drying, electrospinning, bio 3D printing), and strict aseptic treatment and quality testing. These processes ensure high purity, high biological activity, and structural controllability. Importantly, they exhibit good stability and repeatability, with preliminary verification of feasibility for transformation into large-scale, standardized industrial production, laying a solid foundation for future commercialization.

Downloadable Resources:

• Technical White Paper (PDF) (Placeholder)
• Relevant bioRxiv Preprints: (Specific links provided in Medical Applications section)